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Absolute dependence on  mec A gene as the defining standard in determining the resistance of  S. aureus  to methicillin became the subject of distrust by many researchers. The present study aimed to determine the frequency of  mec A gene in methicillin resistant  S. aureus  (MRSA) isolates using polymerase chain reaction and to correlate its presence to conventional method. In this regard, two hundred  S. aureus  isolates were collected from patients with different diseases attending different hospitals in Shandi City, Sudan. Phenotypic Kirby-Bauer method confirmed the existence of methicillin resistant  S. aureus  in 61.5% of the subjected isolates with MICs ranging from 4  μ g/mL to 256  μ g/mL when using  E -test. However, when amplifying a 310 bp fragment of the  mec A gene by PCR, twelve out of the 123 MRSA isolates (9.8%) were  mec A negative, whereas all the 77 methicillin sensitive  S. aureus  (MSSA) were  mec A negative. In conclusion, this study drew attention to the credibility of the  mec A gene and its usefulness in the detection of all MRSA strains without referring to the traditional methods. Hence, it is highly recommended to consider alternative mechanisms for  β -lactam resistance that may compete with  mec A gene in the emergence of MRSA phenomenon in the community.

Absence of themecA Gene in Methicillin ResistantStaphylococcus aureusIsolated from Different Clinical Specimens in Shendi City, Sudan