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The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType  Mycobacterium  CM/AS (common mycobacteria/additional species). A total of 66  Mycobacterium  isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType  Mycobacterium  from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice ( M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum ). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species.

Use of MALDI-TOF MS for Identification of NontuberculousMycobacteriumSpecies Isolated from Clinical Specimens