English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Toxin B is one of the major virulence factors of  Clostridium difficile , a bacterium that is responsible for a significant number of diarrhea cases in acute care settings. Due to the prevalence of  C. difficile  induced diarrhea, rapid and correct diagnosis is crucial in the disease management. In this study, we have employed a stringent  in vitro  selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for toxin B. At the end of the 12-round selection, one MRE with high affinity ( K    d   = 47.3 nM) for toxin B was identified. The selected MRE demonstrated low cross binding activities on negative targets: bovine serum albumin,  Staphylococcus aureus  alpha toxin,  Pseudomonas aeruginosa  exotoxin A, and cholera toxin of  Vibrio cholera . A modified sandwich ELISA assay was developed utilizing the selected ssDNA MRE as the antigen capturing element and achieved a sensitive detection of 50 nM of toxin B in human fecal preparations.

journal of nucleic acids

<i>In Vitro</i>Selection of a Single-Stranded DNA Molecular Recognition Element against<i>Clostridium difficile</i>Toxin B and Sensitive Detection in Human Fecal Matter

In VitroSelection of a Single-Stranded DNA Molecular Recognition Element againstClostridium difficileToxin B and Sensitive Detection in Human Fecal Matter