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. Surgical site infection remains a challenge for hospital infection control, especially when it relates to skin antisepsis in the surgical site.  Objective . To analyze the antimicrobial activity  in vivo  of an antiseptic from ethanol crude extracts of  P. granatum  and  E. uniflora  against Gram-positive and Gram-negative bacteria.  Methods . Agar drilling and minimal inhibitory tests were conducted for  in vitro  evaluation. In the  in vivo  bioassay were used  Wistar  rats and  Staphylococcus aureus  (ATCC 25923) and  Staphylococcus epidermidis  (ATCC 14990). Statistical analysis was performed through variance analysis and Scott-Knott cluster test at 5% probability and significance level.  Results . In the  in vitro , ethanolic extracts of  Punica granatum  and  Eugenia uniflora  and their combination showed the best antimicrobial potential against  S. epidermidis  and  S. aureus . In the  in vivo  bioassay against  S. epidermidis , there was no statistically significant difference between the tested product and the patterns used after five minutes of applying the product.  Conclusion . The results indicate that the originated product is an antiseptic alternative source against  S. epidermidis  compared to chlorhexidine gluconate. It is suggested that further researches are to be conducted in different concentrations of the test product, evaluating its effectiveness and operational costs.

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Antimicrobial Analysis of an Antiseptic Made from Ethanol Crude Extracts ofP. granatumandE. uniflorainWistarRats againstStaphylococcus aureusandStaphylococcus epidermidis