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Ag nanorod arrays prepared by oblique angle vapor deposition (OAD) represent regular, large area substrates for surface-enhanced Raman scattering (SERS) spectroscopy. We studied uniformity and spectral reproducibility of silver OAD-fabricated substrates (AgOADs) by spectral mapping of methylene blue. The results demonstrate good reproducibility apart from occasional “hot-spot” sites where the intensity is higher. The number of “hot-spots” represents 2%–6% of SERS-active sites of mapping substrate area. We were able to obtain good SERS spectra of testing amino acid tryptophan at 1 × 10−5 M concentration and three different free-base porphyrins down to ∼10−7 M concentration. We found out that keeping the AgOADs in a vacuum chamber overnight prevents the surface from binding any contaminants from the ambient atmosphere, without significant reduction in the SERS enhancement. Such substrates provide stable SERS enhancement even when stored for 1 year after preparation

Ag Nanorod Arrays for SERS: Aspects of Spectral Reproducibility, Surface Contamination, and Spectral Sensitivity