English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Cell wall-active antibiotics cause induction of a locus that leads to elevated synthesis of two methionine sulfoxide reductases (MsrA1 and MsrB) in  Staphylococcus aureus . To understand the regulation of this locus, reporter strains were constructed by integrating a DNA fragment consisting of the  msrA1/msrB  promoter in front of a promoterless  lacZ  gene in the chromosome of wild-type and MsrA1-, MsrB-, MsrA1/MsrB-, and SigB-deficient methicillin-sensitive  S. aureus  strain SH1000 and methicillin-resistant  S. aureus  strain COL. These reporter strains were cultured in TSB and the cellular levels of  β -galactosidase activity in these cultures were assayed during different growth phases.  β -galactosidase activity assays demonstrated that the lack of MsrA1, MsrB, and SigB upregulated the  msrA1/msrB  promoter in  S. aureus  strain SH1000. In  S. aureus  strain COL, the highest level of  β -galactosidase activity was observed under the conditions when both MsrA1 and MsrB proteins were absent. The data suggest that the  msrA1/msrB  locus, in part, is negatively regulated by MsrA1, MsrB, and SigB in  S. aureus .

Regulation of Expression of Oxacillin-Inducible Methionine Sulfoxide Reductases inStaphylococcus aureus