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WRKY transcription factor is involved in multiple life activities including plant growth and development as well as biotic and abiotic responses. We identified 28  WRKY  genes from transcriptome data of  Ginkgo biloba  according to conserved WRKY domains and zinc finger structure and selected three WRKY genes, which are  GbWRKY2, GbWRKY16 , and  GbWRKY21 , for expression pattern analysis.  GbWRKY2  was preferentially expressed in flowers and strongly induced by methyl jasmonate. Here, we cloned the full-length cDNA and genomic DNA of  GbWRKY2 . The full-length cDNA of  GbWRKY2  was 1,713 bp containing a 1,014 bp open reading frame encoding a polypeptide of 337 amino acids. The  GbWRKY2  genomic DNA had one intron and two exons. The deduced  GbWRKY2  contained one WRKY domain and one zinc finger motif.  GbWRKY2  was classified into Group II WRKYs. Southern blot analysis revealed that  GbWRKY2  was a single copy gene in  G. biloba . Many  cis -acting elements related to hormone and stress responses were identified in the 1,363 bp-length 5′-flanking sequence of  GbWRKY2 , including W-box, ABRE-motif, MYBCOREs, and PYRIMIDINE-boxes, revealing the molecular mechanism of upregulated expression of  GbWRKY2  by hormone and stress treatments. Further functional characterizations in transiently transformed tobacco leaves allowed us to identify the region that can be considered as the minimal promoter.

Isolation, Expression, and Promoter Analysis ofGbWRKY2: A Novel Transcription Factor Gene fromGinkgo biloba