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A procedure for rapid clonal propagation of  Passiflora edulis  Sims. f.  flavicarpa  Deg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl 2  and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL −1  6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21 ± 1.13) primordial from the nodal meristems. Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL −1  of each BAP and Kinetin (Kin) was successful for the multiplication of the shoots  in vitro  with maximum numbers of shoots (25.73 ± 0.06) within four weeks of incubation. Shoots were rooted best (7.13 ± 0.56 roots/shoots) on half strength MS medium supplemented with 2.0 mgL −1  indole-3 butyric acid (IBA). All  in vitro  regenerated shoots were rooted by  ex vitro  method, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL −1  IBA. The plantlets were hardened in the greenhouse for 4-5 weeks. The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate.

An Improved Micropropagation Protocol byEx VitroRooting ofPassiflora edulisSims. f.flavicarpaDeg. through Nodal Segment Culture