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Mycobacterium bovis  is the major causative agent of bovine tuberculosis, one of the most relevant zoonoses in the world, and affects a wide range of wild and domesticated animals. Development of screening panels in mycobacterial genotyping, according to specific geographical regions, is strongly needed. The aim of this study is to select a panel, constituted by highly polymorphic MIRU-VNTR  loci , to discriminate clinical isolates of  M. bovis  in Mexico. In this study, 65 isolates of  M. bovis  obtained from clinical bovine samples proceeding from different geographic regions of Mexico were identified by phenotypic and genotypic tests and subsequently genotyped by a 24- locus  MIRU-VNTR panel. The most polymorphic  loci  were selected to build a panel with a high discriminatory power similar to the 24- locus  panel results. A panel of seven elements (QUB 11a, MIRU 26, ETR-A, QUB 26, MIRU 16, MIRU 27, and MIRU 39) with the highest allelic diversity showed an appropriate differentiation. The selected MIRU-VNTR elements, according to the regional allelic variability, may be used in the preliminary genotyping of  Mycobacterium bovis  isolates in Mexico.

Proposal of a Screening MIRU-VNTR Panel for the Preliminary Genotyping ofMycobacterium bovisin Mexico