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Purpose . To investigate how multipotent retinal stem cells (RSCs) isolated from mice respond to the proinflammatory signaling molecules, IL-1 β , IL-18, and IL-17A.  Materials and Methods . RSCs were cultured in a specific culture medium and were treated with these cytokines. Cell viability was detected by MTT assay; ultrastructure was evaluated by transmission electron microscopy; expression of IL-17rc and proapoptotic proteins was detected by immunocytochemistry and expression of  Il-6  and  Il-17a  was detected by quantitative RT-PCR. As a comparison, primary mouse retinal pigment epithelium (RPE) cells were also treated with IL-1 β , IL-18, or IL-17A and analyzed for the expression of  Il-6  and  Il-17rc .  Results . Treatment with IL-1 β , IL-18, or IL-17A decreased RSC viability in a dose-dependent fashion and led to damage in cellular ultrastructure including pyroptotic and/or necroptotic cells. IL-1 β  and IL-18 could induce proapoptotic protein expression. All treatments induced significantly higher expression of  Il-6  and  Il-17rc  in both cells. However, neither IL-1 β  nor IL-18 could induce  Il-17a  expression in RSCs.  Conclusions . IL-1 β , IL-18, and IL-17A induce retinal cell death via pyroptosis/necroptosis and apoptosis. They also provoke proinflammatory responses in RSCs. Though IL-1 β  and IL-18 could not induce  Il-17a  expression in RSCs, they both increase  Il-17rc  expression, which may mediate the effect of  Il-17a .

Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17