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A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based on Taqman technology. Assay linearity was confirmed with the serial dilutions of RTA (Turkey) containing 1 × (10 6 , 10 5 , 10 4 , and 10 3 ) IU mL −1 . Comparison of test results obtained by two extraction methods showed a good correlation ( r  = 0.95,  n  = 30) with detection limit of 10 2  IU mL −1 . The semiautomated vacuum filtration based protocol demonstrated high throughput: 35 minutes for the extraction of a batch of 30 samples (150  µ L each) with reduced labor, time, waste, and cost. Performance characteristics of semiautomated system make it suitable for use in diagnostic purpose and viral load determinations.

Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation