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The  Salmonella  enterotoxin ( stn ) gene exhibits high homology among  S. enterica  serovars and  S. bongori . A set of 6 specific primers targeting the  stn  gene were designed for detection of  Salmonella  spp. using the loop-mediated isothermal amplification (LAMP) method. The primers amplified target sequences in all 102 strains of 87 serovars of  Salmonella  tested and no products were detected in 57 non- Salmonella  strains. The detection limit in pure cultures was 5 fg DNA/reaction when amplified at 65°C for 25 min. The LAMP assay could detect  Salmonella  in artificially contaminated food samples as low as 220 cells/g of food without a preenrichment step. However, the sensitivity was increased 100-fold (~2 cells/g) following 5 hr preenrichment at 35°C. The LAMP technique, with a preenrichment step for 5 and 16 hr, was shown to give 100% specificity with food samples compared to the reference culture method in which 67 out of 90 food samples gave positive results. Different food matrixes did not interfere with LAMP detection which employed a simple boiling method for DNA template preparation. The results indicate that the LAMP method, targeting the  stn  gene, has great potential for detection of  Salmonella  in food samples with both high specificity and high sensitivity.

Efficient and Specific Detection ofSalmonellain Food Samples Using astn-Based Loop-Mediated Isothermal Amplification Method