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The aim of this study was to determine the impending antioxidant properties of different extracts of crude methanolic extract (CME) of leaves of  Lannea coromandelica  ( L. coromandelica ) and its two ethyl acetate (EAF) and aqueous (AqF) subfractions by employing various established  in vitro  systems and estimation of total phenolic and flavonoid content. The results showed that extract and fractions possessed strong antioxidant activity  in vitro  and among them, EAF had the strongest antioxidant activity. EAF was confirmed for its highest phenolic content, total flavonoid contents, and total antioxidant capacity. The EAF was found to show remarkable scavenging activity on 2,2-diphenylpicrylhydrazyl (DPPH) (EC 50  63.9 ± 0.64  µ g/mL), superoxide radical (EC 50  8.2 ± 0.12 mg/mL), and Fe 2+  chelating activity (EC 50  6.2 ± 0.09 mg/mL). Based on our  in vitro  results, EAF was investigated for  in vivo  antioxidant assay. Intragastric administration of the EAF can significantly increase levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels, and decrease malondialdehyde (MDA) content in the liver and kidney of CCl 4 -intoxicated rats. These new evidences show that  L. coromandelica  bared antioxidant activity.

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Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of FolkloricLannea coromandelicaUsingIn VitroandIn VivoAssays