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Treatment with 100 mg/kg and 200 mg/kg body weight of aqueous leaf extract (ALE) of  Cardiospermum halicacabum  for 30 days produced a significant dose dependent increase in the sperm counts and sperm motility in both caput and cauda regions. Further, significant increase in serum testosterone level was evident at all applied doses. However, no significant changes in the weight of sex organs were observed. Aqueous leaf extract also increased the number of females impregnated, number of implantations, and number of viable fetuses while decreasing the total number of resorption sites in the pregnant females. However, the total cholesterol level in the serum remained unchanged and there were no records on renotoxicity; nevertheless ALE exhibited a hepatoprotective effect. It was concluded that aqueous leaf extract of  Cardiospermum halicacabum  enhanced sperm concentration, motility, and testosterone, leading to positive results in fertility.

Evaluation of Aqueous Leaf Extract ofCardiospermum halicacabum(L.) on Fertility of Male Rats