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Objective . To investigate the possible mechanisms of oxymatrine's role in anti laryngeal squamous cell carcinoma.  Methods . We examined the effects of oxymatrine on the proliferation, cell cycle phase distribution, apoptosis, and the protein and mRNA expression levels of  HPV16E7  gene in laryngeal carcinoma Hep-2 cells in vitro. The  HPV16E7  siRNA inhibition was also done to confirm the effect of downregulating  HPV16E7  on the proliferation in Hep-2 cells.  Results . Oxymatrine significantly inhibited the growth and proliferation of Hep-2 cells in a dose-dependence and time-dependence manner. Oxymatrine blocked Hep-2 cells in G0/G1 phase, resulting in an obvious accumulation of G0/G1 phase cells while decreasing S phase cells. Oxymatrine induced apoptosis of Hep-2 cells, whose apoptotic rate amounted to about 42% after treatment with 7 mg/mL oxymatrine for 72 h. Oxymatrine also downregulated the expression of  HPV16E7  gene, as determined by the western blotting and reverse transcription-polymerase chain reaction analysis. Knockdown of  HPV16E7  effectively inhibited the proliferation of Hep-2 cells.  Conclusions . Oxymatrine inhibits the proliferation and induces apoptosis of laryngeal carcinoma Hep-2 cells, which might be mediated by a significant cell cycle arrest in G0/G1 phase and downregulation of  HPV16E7  gene. Oxymatrine is considered to be a likely preventive and curative candidate for laryngeal cancer.

Oxymatrine DownregulatesHPV16E7Expression and Inhibits Cell Proliferation in Laryngeal Squamous Cell Carcinoma Hep-2 Cells In Vitro