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Plants from Gynura family was used in this study, namely,  Gynura procumbens  and  Gynura bicolor .  Gynura procumbens  is well known for its various medicinal properties such as antihyperglycaemic, antihyperlipidaemic, and antiulcerogenic; meanwhile,  G. bicolor  remains unexploited. Several nonenzymatic antioxidants methods were utilized to study the antioxidant capacity, which include ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, total flavonoid content, total phenolic content, and ascorbic acid content determination. DPPH assay reveals  G. procumbens  shoot as the lowest (66.885%) and  G. procumbens  root as the highest (93.499%) DPPH radical inhibitor. In FRAP assay, reducing power was not detected in  G. procumbens  leaf callus (0.000 TEAC mg/g FW) whereby  G. procumbens  root exhibits the highest (1.103 TEAC mg/g FW) ferric reducing power. Total phenolic content and total flavonoid content exhibited similar trend for both the intact plants analysed. In all antioxidant assays,  G. procumbens  callus culture exhibits very low antioxidant activity. However,  G. procumbens  root exhibited highest phenolic content, flavonoid content, and ascorbic acid content with 4.957 TEAC mg/g FW, 543.529 QE  µ g/g FW, and 54.723  µ g/g FW, respectively. This study reveals that  G. procumbens  root extract is a good source of natural antioxidant.

Antioxidant Potential in Different Parts and Callus ofGynura procumbensand Different Parts ofGynura bicolor