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Aim . Our aim the was to screen the commonly used Chinese herbs in order to detect changes in  ERBB2  and  ESR1  gene expression using MCF-7 cells.  Methods . Using the MCF-7 human breast cancer cell line, cell cytotoxicity and proliferation were evaluated by MTT and trypan blue exclusion assays, respectively. A luciferase reporter assay was established by transient transfecting MCF-7 cells with plasmids containing either the  ERBB2  or the  ESR1  promoter region linked to the luciferase gene. Chinese herbal extracts were used to treat the cells at 24 h after transfection, followed by measurement of their luciferase activity. The screening results were verified by Western blotting to measure HER2 and ER  α   protein expression.  Results . At concentrations that induced little cytotoxicity, thirteen single herbal extracts and five compound recipes were found to increase either  ERBB2  or  ESR1  luciferase activity. By Western blotting, Si-Wu-Tang, Kuan-Shin-Yin, and Suan-Tsao-Ren-Tang were found to increase either HER2 or ER  α   protein expression. In addition,  Ligusticum chuanxiong  was shown to have a great effect on  ERBB2  gene expression and synergistically with estrogen to stimulate MCF-7 cell growth.  Conclusion . Our results provide important information that should affect clinical treatment strategies among breast cancer patients who are receiving hormonal or targeted therapies.

Screening to Identify Commonly Used Chinese Herbs That AffectERBB2andESR1Gene Expression Using the Human Breast Cancer MCF-7 Cell Line