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The  in vitro  virustatic and virucidal tests of the crude extract of  Cynodon dactylon  against infection with porcine reproductive and respiratory syndrome virus (PRRSV), a cause of major devastating pig disease, were described. Crude extract of  C. dactylon  was prepared for cytotoxicity on tissue-culture cells that were used to measure virustatic and virucidal activities against PRRSV. Crude extract of  C. dactylon  at 0.78 mg/mL showed no cytotoxicity on the cell line, and at that concentration significantly inhibited replication of PRRSV as early as 24 hours post infection (hpi).  C. dactylon  also inactivated PRRSV as determined by immunoperoxidase monolayer assay (IPMA) compared to the control experiments. In summary, the present study may be among the earliest studies to describe virustatic and virucidal activities of  C. dactylon  crude extract against PRRSV  in vitro . Extracts of  C. dactylon  may be useful for PRRSV control and prevention on pig farms.

veterinary medicine international

<i>In Vitro</i>Virucidal and Virustatic Properties of the Crude Extract of<i>Cynodon dactylon</i>against Porcine Reproductive and Respiratory Syndrome Virus

In VitroVirucidal and Virustatic Properties of the Crude Extract ofCynodon dactylonagainst Porcine Reproductive and Respiratory Syndrome Virus