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Large bone defects are a considerable challenge to reconstructive surgeons. Numerous traditional Chinese herbal medicines have been used to repair and regenerate bone tissue. This study investigated the bone regeneration potential of  Danggui Buxue Tang  ( DBT ), a Chinese herbal decoction prepared from  Radix Astragali  ( RA ) and  Radix Angelicae Sinensis  ( RAS ), from a molecular biology perspective. The optimal ratio of  RA  and  RAS  used in  DBT  for osteoblast culture was obtained by colorimetric and alkaline phosphatase (ALP) activity assays. Moreover, the optimal concentration of  DBT  for bone cell culture was also determined by colorimetric, ALP activity, nodule formation, Western blotting, wound-healing, and tartrate-resistant acid phosphatase activity assays. Consequently, the most appropriate weight ratio of  RA  to  RAS  for the proliferation and differentiation of osteoblasts was 5 : 1. Moreover, the most effective concentration of  DBT  was 1,000  μ g/mL, which significantly increased the number of osteoblasts, intracellular ALP levels, and nodule numbers, while inhibiting osteoclast activity. Additionally, 1,000  μ g/mL of  DBT  was able to stimulate p-ERK and p-JNK signal pathway. Therefore,  DBT  is highly promising for use in accelerating fracture healing in the middle or late healing periods.

Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal DecoctionDanggui Buxue Tangfrom a Molecular Biology Perspective