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Ten extracts with different polarity from two Iranian  Artemisia  species,  A. armeniaca  Lam. and  A. aucheri  Boiss, were screened for their antimalarial properties by  in vitro      β  -hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC 50  values of 1.36 ± 0.01 and 1.83 ± 0.03 mg/mL and IC 90  values of 2.12 ± 0.04 and 2.62 ± 0.09 mg/mL for  A. armeniaca  and  A. aucheri , respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of  A. armeniaca  and four fractions from DCM extract of  A. aucheri  showed potent antimalarial activity with reducing IC 50  and IC 90  values compared to extracts. The most potent fraction belonged to DCM extract of  A. armeniaca  with IC 50  and IC 90  values of 0.47 ± 0.006 and 0.71 ± 0.006 mg/mL, respectively.

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Evaluation ofIn VitroAntimalarial Activity of Different Extracts ofArtemisia aucheriBoiss. andA. armeniacaLam. and Fractions of the Most Potent Extracts