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In vitro  direct regeneration of  Nelumbo nucifera  Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under  in vitro  conditions.

the scientific world journal/thescientificworldjournal

Effects of NAA and BAP, Double-Layered Media, and Light Distance on<i>In Vitro</i>Regeneration of<i>Nelumbo nucifera</i>Gaertn. (Lotus), an Aquatic Edible Plant

Effects of NAA and BAP, Double-Layered Media, and Light Distance onIn VitroRegeneration ofNelumbo nuciferaGaertn. (Lotus), an Aquatic Edible Plant