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In the present study we examined whether LPA can be synthesized and act during  in vitro  maturation of bovine cumulus oocyte complexes (COCs). We found transcription of genes coding for enzymes of LPA synthesis pathway ( ATX  and  PLA2 ) and of LPA receptors ( LPAR 1–4 ) in bovine oocytes and cumulus cells, following  in vitro  maturation. COCs were matured  in vitro  in presence or absence of LPA (10 −5  M) for 24 h. Supplementation of maturation medium with LPA increased mRNA abundance of  FST  and  GDF9  in oocytes and decreased mRNA abundance of  CTSs  in cumulus cells. Additionally, oocytes stimulated with LPA had higher transcription levels of  BCL2  and lower transcription levels of  BAX  resulting in the significantly lower  BAX/BCL2  ratio. Blastocyst rates on day 7 were similar in the control and the LPA-stimulated COCs. Our study demonstrates for the first time that bovine COCs are a potential source and target of LPA action. We postulate that LPA exerts an autocrine and/or paracrine signaling, through several LPARs, between the oocyte and cumulus cells. LPA supplementation of maturation medium improves COC quality, and although this was not translated into an enhanced  in vitro  development until the blastocyst stage, improved oocyte competence may be relevant for subsequent  in vivo  survival.

mediators of inflammation

The Effect of Lysophosphatidic Acid during<i>In Vitro</i>Maturation of Bovine Oocytes: Embryonic Development and mRNA Abundances of Genes Involved in Apoptosis and Oocyte Competence

The Effect of Lysophosphatidic Acid duringIn VitroMaturation of Bovine Oocytes: Embryonic Development and mRNA Abundances of Genes Involved in Apoptosis and Oocyte Competence