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Artemisia judaica  L. is a medicinal plant that is traditionally used to relieve abdominal pains through its carminative activity. In this study, spectroscopic analysis was employed to investigate the carminative activity associated with  A. judaica . Using infrared spectroscopy, the carminative activity was evaluated based on the first derivative of IR-characteristic stretching signal of CO 2 . Our results indicate that  A. judaica  oil effectively reduced the response of CO 2  signal equivalent to thymol standard. Additionally,  1 H-NMR spectroscopy was utilized to assess surface activity of  A. judaica  crude oil through the reduction of interfacial tension in a D 2 O/CDCl 3  system. Apparently, 10 mg of the oil was able to solubilize water in a chloroform layer up to 4.3% (w/w). In order to correlate the observed surface activity of the oil to its actual composition, GC-MS and GC-FID structural analysis were undertaken. The results revealed that the oil composition consists of oxygenated terpenes which might be responsible for the carminative effect. Furthermore, owing to its sensitivity, our model provides a fundamental basis for the pharmacological assessment of trace amounts of oils with high precision and accuracy.

The Development and Application of Novel IR and NMR-Based Model for the Evaluation of Carminative Effect ofArtemisia judaicaL. Essential Oil