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This research aimed to develop an electrolysis method to generate high-concentration chlorine dioxide (ClO 2 ) for tilapia fillet disinfection. The designed generator produced up to 3500 ppm of ClO 2  at up to 99% purity. Tilapia fillets were soaked in a 400 ppm ClO 2  solution for 5, 10, and 25 min. Results show that total plate counts of tilapia, respectively, decreased by 5.72 to 3.23, 2.10, and 1.09 log CFU/g. In addition, a 200 ppm ClO 2  solution eliminated coliform bacteria and  Escherichia coli  in 5 min with shaking treatment. Furthermore, ClO 2  and trihalomethanes (THMs) residuals on tilapia fillets were analyzed by GC/MS and were nondetectable (GC-MS detection limit was 0.12 ppb). The results conform to Taiwan's environmental protection regulations and act governing food sanitation.

Application of Highly Purified Electrolyzed Chlorine Dioxide for Tilapia Fillet Disinfection