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Optimization of workflow for breast cancer samples with equivocal HER2/neu score 2+ results in routine practice remains to be a central focus of the ongoing efforts to assess HER2 status. According to the College of American Pathologists/American Society of Clinical Oncology guidelines equivocal HER2/neu score 2+ cases are subject for further testing, usually by fluorescence in situ hybridization (FISH) investigations. It still remains on open question, whether quantitative digital image analysis of HER2 immunohistochemically (IHC) stained slides can assist in further refining the HER2 score 2+.

Comparative Study between Quantitative Digital Image Analysis and Fluorescence In Situ Hybridization of Breast Cancer Equivocal HER2 2+ Cases