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A new stackable modular system was developed for continuous in vivo production of phytoseiid mites. The system consists of cage units that are filled with lima beans, Phaseolus lunatus, or red beans, P. vulgaris, leaves infested with high levels of the two-spotted spider mites, Tetranychus urticae. The cage units connect with each other through a connection cup, which also serves for monitoring and collection. Predatory mites migrate upwards to new cage units as prey is depleted. The system was evaluated for production of Phytoseiulus persimilis. During a 6-month experimental period, 20,894.9 ± 10,482.5 (mean ± standard deviation) predators were produced per week. The production consisted of 4.1 ± 4.6 % nymphs and 95.9 ± 4.6 % adults. A mean of 554.5 ± 59.8 predatory mites were collected per harvested cage and the mean interval length between harvests was 6.57 ± 6.76 days. The potential for commercial and experimental applications is discussed.

A Modular Cage System Design for Continuous Medium to Large Scale In Vivo Rearing of Predatory Mites (Acari: Phytoseiidae)