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The presence of gene 16S rRNA and genes encoding toxin A ( tcdA ), toxin B ( tcdB ), and binary toxin ( cdtA/cdtB ) of  Clostridium difficile  in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of  tcdA+/tcdB−  and  cdtA+/cdtB+  was verified in one  C. difficile -positive diarrhea sample and of  tcdA+/tcdB+  in three  C. difficile -positive nondiarrhea samples. The presence of  tcdA+/tcdB+  in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains.

Genes Encoding Toxin ofClostridium difficilein Children with and without Diarrhea