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Cryptosporidium  spp.,  Giardia  spp., and members of Microsporidia are enteropathogenic parasites of humans and animals, producing asymptomatic to severe intestinal infections. To circumvent various impediments associated with current detection methods, we tested a method providing multistage purification and separation in a single, confined step. Standard real-time PCR was used as a detection method. Samples spiked with  C. parvum  and  G. intestinalis  were split for comparison to standard Method 1623. Results were equivalent to immunomagnetic procedures for  Cryptosporidium , and  Giardia . Overall percent recovery for  Cryptosporidium  with Method 1623 averaged 26.89% (std 21.44%; min = 0%; max = 73%) and was similar but less variable for qPCR method at an estimated average of 27.67 (std 17.65%; min = 5%; max = 63%). For  Giardia , Method 1623 had an overall average recovery of 27.11% (std 17.98%; min = 1%; max = 58%), while multistage purification and qPCR had an estimated lower overall recovery at 18.58% (std 13.95%; min = 0%; max = 35%). Microsporidia were also readily detected with an estimated recovery of 46.81% overall (std 17.66%; min = 18%; max = 70%) for  E. intestinalis  and 38.90% (std 14.36%; min = 13%; max = 62%) for  E. bieneusi .

Effective Concentration and Detection of Cryptosporidium, Giardia, and the Microsporidia from Environmental Matrices