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A split plot 3 by 4 experiment was designed to investigate and distinguish the relationships among production of secondary metabolites, soluble sugar, phenylalanine ammonia lyase (PAL; EC 4.3.1.5) activity, leaf gas exchange, chlorophyll content, antioxidant activity (DPPH), and lipid peroxidation under three levels of CO 2  (400, 800, and 1200   μ  mol/mol) and four levels of light intensity (225, 500, 625, and 900   μ  mol/m 2 /s) over 15 weeks in  Labisia pumila . The production of plant secondary metabolites, sugar, chlorophyll content, antioxidant activity, and malondialdehyde content was influenced by the interactions between CO 2  and irradiance. The highest accumulation of secondary metabolites, sugar, maliondialdehyde, and DPPH activity was observed under CO 2  at 1200   μ  mol/mol + light intensity at 225   μ  mol/m 2 /s. Meanwhile, at 400   μ  mol/mol CO 2  + 900   μ  mol/m 2 /s light intensity the production of chlorophyll and maliondialdehyde content was the highest. As CO 2  levels increased from 400 to 1200   μ  mol/mol the photosynthesis, stomatal conductance,  f    v  / f    m   (maximum efficiency of photosystem II), and PAL activity were enhanced. The production of secondary metabolites displayed a significant negative relationship with maliondialdehyde indicating lowered oxidative stress under high CO 2  and low irradiance improved the production of plant secondary metabolites that simultaneously enhanced the antioxidant activity (DPPH), thus improving the medicinal value of  Labisia pumila  under this condition.

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Allocation of Secondary Metabolites, Photosynthetic Capacity, and Antioxidant Activity of Kacip Fatimah (<i>Labisia pumila</i>Benth) in Response to<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mtext>C</mml:mtext><mml:msub><mml:mrow><mml:mtext>O</mml:mtext></mml:mrow><mml:mrow><mml:mtext>2</mml:mtext></mml:mrow></mml:msub></mml:math>and Light Intensity

Allocation of Secondary Metabolites, Photosynthetic Capacity, and Antioxidant Activity of Kacip Fatimah (Labisia pumilaBenth) in Response toCO2and Light Intensity