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Ethanol extracts of gymnema ( Gymnema sylvestre ) leaves exhibited marked antitumour-promoting activity in an  in vivo  two-stage carcinogenesis test in mice using 7,12-dimethylbenz[ a ]anthracene as an initiator and 12- O -tetradecanoylphorbol-13-acetate (TPA) as a promoter. From the active fraction of the ethanol extract of the gymnema leaves, three triterpenoids were isolated and identified. These compounds were evaluated for their inhibitory effects on TPA-induced inflammation (1 µg/ear) in mice. The tested compounds showed marked anti-inflammatory effects, with a 50% inhibitory dose of 50–555 nmol/ear.

Inhibitory Effects of Gymnema (Gymnema sylvestre) Leaves on Tumour Promotion in Two-Stage Mouse Skin Carcinogenesis