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Various explants (stem, leaf, and root) of  Citrus assamensis  were cultured on MS media supplemented with various combinations and concentrations (0.5–2.0 mgL −1 ) of NAA and BAP. Optimum shoot and root regeneration were obtained from stem cultures supplemented with 1.5 mgL −1  NAA and 2.0 mgL −1  BAP, respectively. Explant type affects the success of tissue culture of this species, whereby stem explants were observed to be the most responsive. Addition of 30 gL −1  sucrose and pH of 5.8 was most optimum for   in vitro  regeneration of this species. Photoperiod of 16 hours of light and 8 hours of darkness was most optimum for shoot regeneration, but photoperiod of 24 hours of darkness was beneficial for production of callus. The morphology (macro and micro) and anatomy of   in vivo  and   in vitro / ex vitro Citrus assamensis  were also observed to elucidate any irregularities (or somaclonal variation) that may arise due to tissue culture protocols. Several minor micromorphological and anatomical differences were observed, possibly due to stress of tissue culture, but   in vitro  plantlets are expected to revert back to normal phenotype following full adaptation to the natural environment.

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Optimization of Culture Conditions (Sucrose, pH, and Photoperiod) for<i>In Vitro</i>Regeneration and Early Detection of Somaclonal Variation in Ginger Lime (<i>Citrus assamensis</i>)

Optimization of Culture Conditions (Sucrose, pH, and Photoperiod) forIn VitroRegeneration and Early Detection of Somaclonal Variation in Ginger Lime (Citrus assamensis)