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In  Drosophila , protein trap strategies provide powerful approaches for the generation of tagged proteins expressed under endogenous control. Here, we describe expression and functional analysis to evaluate new  Ubx  and  hth  protein trap lines generated by the Cambridge Protein Trap project. Both protein traps exhibit spatial and temporal expression patterns consistent with the reported endogenous pattern in the embryo. In imaginal discs, Ubx-YFP is expressed throughout the haltere and 3rd leg imaginal discs, while Hth-YFP is expressed in the proximal regions of haltere and wing discs but not in the pouch region. The  Ubx    CPTI000601   line is semilethal as a homozygote. No T3/A1 to T2 transformations were observed in the embryonic cuticle or the developing midgut. The homozygous survivors, however, exhibit a weak haltere phenotype with a few wing-like marginal bristles on the haltere capitellum. Although  hth    CPTI000378   is completely lethal as a homozygote, the  hth    CPTI000378  / hth    C1   genotype is viable. Using a hth deletion  (Df(3R)BSC479)  we show that  hth    CPTI000378  / Df(3R)BSC479  adults are phenotypically normal. No transformations were observed in  hth    CPTI000378  ,  hth    CPTI000378  / hth    C1  , or  hth    CPTI000378  / Df(3R)BSC479  embryonic cuticles. We have successfully characterised the Ubx-YFP and Hth-YFP protein trap lines demonstrating that the tagged proteins show appropriate expression patterns and produce at least partially functional proteins.

Characterisation ofDrosophila UbxCPTI000601andhthCPTI000378Protein Trap Lines

Characterisation ofDrosophila Ubx^CPTI000601andhth^CPTI000378Protein Trap Lines