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Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado ( Persea americana  var.  drymifolia ) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin  PaDef  cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against  Escherichia coli ,  Staphylococcus aureus , and  Candida albicans .  E. coli  viability was inhibited with 100   μ  g/mL of total protein from clones (>55%). Also,  S. aureus  viability was inhibited from 50   μ  g/mL total protein (27–38%) but was more evident at 100   μ  g/mL (52–65%). This inhibition was higher than the effect showed by polyclonal population ( ~ 23%). Finally, we did not detect activity against  C. albicans . These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.

biomed research international

Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americanavar.drymifolia) Expressed in Endothelial Cells againstEscherichia coliandStaphylococcus aureus