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Mixotrophic metabolism was evaluated as an option to augment the growth and lipid production of marine microalga  Tetraselmis  sp. FTC 209. In this study, a five-level three-factor central composite design (CCD) was implemented in order to enrich the W-30 algal growth medium. Response surface methodology (RSM) was employed to model the effect of three medium variables, that is, glucose (organic C source), NaNO 3  (primary N source), and yeast extract (supplementary N, amino acids, and vitamins) on biomass concentration,  X   max , and lipid yield,  P   max / X   max . RSM capability was also weighed against an artificial neural network (ANN) approach for predicting a composition that would result in maximum lipid productivity, Pr lipid . A quadratic regression from RSM and a Levenberg-Marquardt trained ANN network composed of 10 hidden neurons eventually produced comparable results, albeit ANN formulation was observed to yield higher values of response outputs. Finalized glucose (24.05 g/L), NaNO 3  (4.70 g/L), and yeast extract (0.93 g/L) concentration, affected an increase of  X   max  to 12.38 g/L and lipid a accumulation of 195.77 mg/g dcw. This contributed to a lipid productivity of 173.11 mg/L per day in the course of two-week cultivation.

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Comparative Analyses of Response Surface Methodology and Artificial Neural Network on Medium Optimization for<i>Tetraselmis</i>sp. FTC209 Grown under Mixotrophic Condition

Comparative Analyses of Response Surface Methodology and Artificial Neural Network on Medium Optimization forTetraselmissp. FTC209 Grown under Mixotrophic Condition