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In this study dairy sludge was evaluated as an alternative cultivation medium for  Rhizobium . Growth of bacterial strains at different concentrations of Dairy sludge was monitored. Maximum growth of all strains was observed at 60% Dairy sludge concentration. At 60% optical density (OD) values are 0.804 for  Rhizobium trifolii  (MTCC905), 0.825 for  Rhizobium trifolii  (MTCC906), and 0.793 for  Rhizobium meliloti  (MTCC100). Growth pattern of strains was observed at 60% Dairy sludge along with different synthetic media (tryptone yeast,  Rhizobium  minimal medium and yeast extract mannitol). Growth in 60% Dairy sludge was found to be superior to standard media used for  Rhizobium . Media were optimized using 60% dairy sludge along with different concentrations of yeast extract (1–7 g/L) and mannitol (7–13 g/L) in terms of optical density at different time intervals, that is, 24, 48 and 72 hours. Maximum growth was observed in 6 g/L of yeast extract and 12 g/L of mannitol at 48-hour incubation period in all strains. The important environmental parameters such as pH were optimized using 60% dairy sludge, 60% dairy sludge +6 g/L yeast extract, and 60% dairy sludge +12 g/L mannitol. The maximum growth of all strains was found at pH 7.0. The present study recommends the use of 60% dairy sludge as a suitable growth medum for inoculant production.

Optimization of Dairy Sludge for Growth ofRhizobiumCells