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One hundred and twenty-five acid-resistant presumptive lactobacilli were isolated from Slovak Bryndza cheese and screened for their antimicrobial activity against eight bacterial pathogens using spot agar assay. Out of twenty-six  Lactobacillus  strains with strong inhibition activity, twenty were identified as  Lactobacillus plantarum  and six as  Lactobacillus fermentum . The most active eleven  L. plantarum  isolates were further characterized  in vitro  for some probiotic and safety properties. Only three isolates K10, K21, and ZS07 showed the ability to grow over 50% in the presence of 0.3% bile. Strong deconjugation efficiency was determined for CK06 and K21. The highest   β  -galactosidase activity was shown in isolates ZS11, B01, CK06, and ZS07. Only three of the strains had the ability to produce tyramine: CK06, LM1, and ZS11. Strains K09, K21, ZS11, and ZS15 were susceptible to all tested antibiotics. Analysis of the results confirmed the  L. plantarum  isolates ZS07 and K21 as the most suitable for probiotic use, due to their desirable probiotic and safety characteristics.

Probiotic Potential and Safety Properties ofLactobacillus plantarumfrom Slovak Bryndza Cheese