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The present study on  Phyllanthus virgatus , known traditionally for its remedial potential, for the first time provides descriptions of the antioxidant and inhibition of   α  -amylase enzyme activity first by  in vitro  analyses, followed by a confirmatory  in silico  study to create a stronger biochemical rationale. Our results illustrated that  P. virgatus  methanol extract exhibited strong antioxidant and oxidative DNA damage protective activity than other extracts, which was well correlated with its total phenolic content. In addition,  P. virgatus  methanol extract strongly inhibited the   α  -amylase activity (IC 50  33.20 ± 0.556   μ  g/mL), in a noncompetitive manner, than acarbose (IC 50  76.88 ± 0.277   μ  g/mL), which showed competitive inhibition. Moreover, this extract stimulated the glucose uptake activity in 3T3-L1 cells and also showed a good correlation between antioxidant and   α  -amylase activities. The molecular docking studies of the major bioactive compounds (9,12-octadecadienoic acid, asarone, 11-octadecenoic acid, and acrylic acid) revealed via GC-MS analysis from this extract mechanistically suggested that the inhibitory property may be due to the synergistic effect of these bioactive compounds. These results provide substantial basis for the future use of  P. virgatus  methanol extract and its bioactive compound in  in vivo  system for the treatment and management of diabetes as well as in the related condition of oxidative stress.

Antioxidant andα-Amylase Inhibitory Property ofPhyllanthus virgatusL.: AnIn Vitroand Molecular Interaction Study