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Streptomyces misionensis  strain PESB-25 was screened and selected for its ability to secrete cellulases. Cells were grown in a liquid medium containing sugarcane bagasse (SCB) as carbon source and corn steep liquor (CSL) as nitrogen source, whose concentrations were optimized using response surface methodology (RSM). A peak of endoglucanase accumulation (1.01 U · mL −1 ) was observed in a medium with SCB 1.0% (w/v) and CSL 1.2% (w/v) within three days of cultivation.  S. misionensis  PESB-25 endoglucanase activity was thermoacidophilic with optimum pH and temperature range of 3.0 to 3.6 and 62° to 70°C, respectively. In these conditions, values of 1.54 U mL −1  of endoglucanase activity were observed. Moreover, Mn 2+  was demonstrated to have a hyperactivating effect on the enzyme. In the presence of MnSO 4  (8 mM), the enzyme activity increased threefold, up to 4.34 U · mL −1 . Mn 2+  also improved endoglucanase stability as the catalyst retained almost full activity upon incubation at 50°C for 4 h, while in the absence of Mn 2+ , enzyme activity decreased by 50% in this same period. Three protein bands with endoglucanase activity and apparent molecular masses of 12, 48.5 and 119.5 kDa were detected by zymogram.

biomed research international

<i>Streptomyces misionensis</i>PESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates

Streptomyces misionensisPESB-25 Produces a Thermoacidophilic Endoglucanase Using Sugarcane Bagasse and Corn Steep Liquor as the Sole Organic Substrates