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The present study aims to investigate the probiotic nature of  Pediococcus acidilactici  MTCC5101 by an  in vitro  assay of bacterial adherence to intestinal epithelial cells of human gastrointestinal (GI) tract using Caco-2 cell line. Further to assess the  in vivo  survival in the GI tract, oral feeding was carried out with the help of 10 healthy volunteers. The effect on wellness was assessed by studying blood biochemical parameters of the volunteers. The survival of the bacteria was assessed using PCR-based detection of  P. acidilactici  MTCC5101 in fecal samples. The probiotic nature of  P. acidilactici  MTCC 5101 was strengthened by its adherence to the intestinal epithelial Caco-2 cell line in the  in vitro  SEM observations. Oral feeding study for assessing the survival of bacteria in GI tract of volunteers showed the strain to be established in the GI tract which survived for about 2 weeks after feeding.

In VitroandIn VivoSurvival and Colonic Adhesion ofPediococcus acidilacticiMTCC5101 in Human Gut