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The  in vitro  cytotoxicity tests on the extracts of  Mesua beccariana ,  M. ferrea , and  M. congestiflora  against Raji, SNU-1, HeLa, LS-174T, NCI-H23, SK-MEL-28, Hep-G2, IMR-32, and K562 were achieved using MTT assay. The methanol extracts of  Mesua beccariana  showed its potency towards the proliferation of B-lymphoma cell (Raji). In addition, only the nonpolar to semipolar extracts (hexane to ethyl acetate) of the three  Mesua  species indicated cytotoxic effects on the tested panel of human cancer cell lines. Antioxidant assays were evaluated using DPPH scavenging radical assay and Folin-Ciocalteu method. The methanol extracts of  M. beccariana  and  M. ferrea  showed high antioxidant activities with low EC 50  values of 12.70 and 9.77   μ  g/mL, respectively, which are comparable to that of ascorbic acid (EC 50  = 5.62   μ  g/mL). Antibacterial tests were carried out using four Gram positive and four Gram negative bacteria on  Mesua beccariana  extracts. All the extracts showed negative results in the inhibition of Gram negative bacteria. Nevertheless, methanol extracts showed some activities against Gram positive bacteria which are  Bacillus cereus , methicillin-sensitive  Staphylococcus aureus  (MSSA), and methicillin-resistant  Staphylococcus aureus  (MRSA), while the hexane extract also contributed some activities towards  Bacillus cereus .

biomed research international

In VitroCytotoxic, Antioxidant, and Antimicrobial Activities ofMesua beccariana(Baill.) Kosterm.,Mesua ferreaLinn., andMesua congestifloraExtracts