Open AccessThe Cellient System for Paraffin Histology Can Be Combined with HPV Testing and Morphotyping the Vaginal Microbiome Thanks to BoonFixing
Author(s) -
Mathilde E. Boon
Publication year - 2013
Publication title -
obstetrics and gynecology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.648
H-Index - 13
eISSN - 1687-9597
pISSN - 1687-9589
DOI - 10.1155/2013/502357
Subject(s) - medicine , ascus (bryozoa) , lesion , histology , fixative , pathology , squamous intraepithelial lesion , cervical cancer , staining , cancer , biology , cervical intraepithelial neoplasia , microbiology and biotechnology , ascospore , spore
The Cellient Automated Cell Block System (Hologic) can be used to process cervical scrapes to paraffin sections. For the first study on this subject, cervical scrapes were fixed in the formalin-free fixative BoonFix. This pilot study was limited to cases classified as atypical squamous lesion of unknown significance (ASCUS) and high-grade squamous lesion (HSIL) as diagnosed in the ThinPrep slide. The Cellient paraffin sections were classified into negative, atypical, CIN 1, CIN 2, and CIN 3. Multiple HPV genotypes were encountered in 79% of the scrapes. This study showed that the Cellient system for paraffin sections can be combined with HPV testing thanks to the formalin-free BoonFix. In two additional studies it was shown that such samples can also be used for morphotyping the vaginal microbiome and preparing cytologic ThinPrep slides.
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