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Background .  gcrR  gene acts as a negative regulator related to sucrose-dependent adherence in  S. mutans . It is constructive to test the potential capacity of mutans with  gcrR  gene deficient in bacteria replacement therapy.  Methods . In this study, we constructed the mutant by homologous recombination. The morphological characteristics of biofilms were analyzed by confocal laser scanning microscopy.  S. mutans  UA159 and the mutant MS-gcrR-def were inoculated, respectively, or together for competitive testing in vitro and in rat model.  Results . Adhesion assay showed that the adhesion ability of the mutant increased relative to the wild type, especially in the early stage. MS-gcrR-def out-competed  S. mutans  UA159 in vitro biofilm, and correspondingly coinfection displayed significantly fewer caries in vivo. The former possessed both a lower level of acid production and a stronger colonization potential than  S. mutans  UA159.  Conclusion . These findings demonstrate that MS-gcrR-def appears to be a good candidate for replacement therapy.

A New gcrR-DeficientStreptococcus mutansMutant for Replacement Therapy of Dental Caries