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The  in vitro  antibacterial and antibiofilm activity of chlorogenic acid against clinical isolates of  Stenotrophomonas maltophilia  was investigated through disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), time-kill and biofilm assays. A total of 9 clinical  S. maltophilia  isolates including one isolate resistant to trimethoprim/sulfamethoxazole (TMP/SMX) were tested. The inhibition zone sizes for the isolates ranged from 17 to 29 mm, while the MIC and MBC values ranged from 8 to 16   μ  g mL −1  and 16 to 32   μ  g mL −1 . Chlorogenic acid appeared to be strongly bactericidal at 4x MIC, with a 2-log reduction in viable bacteria at 10 h.  In vitro  antibiofilm testing showed a 4-fold reduction in biofilm viability at 4x MIC compared to 1x MIC values (0.085 < 0.397 A 490 nm) of chlorogenic acid. The data from this study support the notion that the chlorogenic acid has promising  in vitro  antibacterial and antibiofilm activities against  S. maltophilia .

biomed research international

<i>In Vitro</i>Antibacterial and Antibiofilm Activities of Chlorogenic Acid against Clinical Isolates of<i>Stenotrophomonas maltophilia</i>including the Trimethoprim/Sulfamethoxazole Resistant Strain

In VitroAntibacterial and Antibiofilm Activities of Chlorogenic Acid against Clinical Isolates ofStenotrophomonas maltophiliaincluding the Trimethoprim/Sulfamethoxazole Resistant Strain