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Current study evaluated the  hsp65  Nested PCR Restriction Fragment Length Polymorphism Analysis ( hsp65  Nested PCR-PRA) to detect and identify  Mycobacterium tuberculosis  complex directly in clinical samples for a rapid and specific diagnosis of tuberculosis (TB).  hsp65  Nested PCR-PRA was applied directly to 218 clinical samples obtained from 127 patients suspected of TB or another mycobacterial infection from July 2009 to July 2010. The  hsp65  Nested PCR-PRA showed 100% sensitivity and 95.0 and 93.1% specificity in comparison with culture and microscopy (acid fast bacillus smear), respectively.  hsp65  Nested PCR-PRA was shown to be a fast and reliable assay for diagnosing TB, which may contribute towards a fast diagnosis that could help the selection of appropriate chemotherapeutic and early epidemiological management of the cases which are of paramount importance in a high TB burden country.

Evaluation ofhsp65Nested PCR-Restriction Analysis (PRA) for Diagnosing Tuberculosis in a High Burden Country