English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

N   α   -terminal acetylation of peptides plays an important biological role but is rarely observed in prokaryotes. N   α   -terminal acetylated thymosin   α  1 (T  α  1), a 28-amino-acid peptide, is an immune modifier that has been used in the clinic to treat hepatitis B and C virus (HBV/HCV) infections. We previously documented N   α   -terminal acetylation of recombinant prothymosin   α   (ProT  α  ) in  E. coli . Here we present a method for production of N   α   -acetylated T  α  1 from recombinant ProT  α  . The recombinant ProT  α   was cleaved by human legumain expressed in  Pichia pastoris  to release T  α  1  in vitro . The N   α   -acetylated T  α  1 peptide was subsequently purified by reverse phase and cation exchange chromatography. Mass spectrometry indicated that the molecular mass of recombinant N   α   -acetylated T  α  1 was 3108.79 in, which is identical to the mass of N   α   -acetylated T  α  1 produced by total chemical synthesis. This mass corresponded to the nonacetylated T  α  1 mass with a 42 Da increment. The retention time of recombinant N   α   -acetylated T  α  1 and chemosynthetic N   α   -acetylated T  α  1 were both 15.4 min in RP-high performance liquid chromatography (HPLC). These data support the use of an  E. coli  expression system for the production of recombinant human N   α   -acetylated T  α  1 and also will provide the basis for the preparation of recombinant acetylated peptides in  E. coli .

Generation of Mature Nα-Terminal Acetylated Thymosinα1 by Cleavage of Recombinant Prothymosinα

Generation of Mature N^α-Terminal Acetylated Thymosinα1 by Cleavage of Recombinant Prothymosinα