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Osmoregulated periplasmic glucans (OPGs) are oligosaccharides found in the periplasm of many Gram-negative bacteria. Glucose is the sole constitutive sugar and this backbone may be substituted by various kinds of molecules depending on the species. In  E. coli , OPG are substituted by phosphoglycerol and phosphoethanolamine derived from membrane phospholipids and by succinyl residues. In this study, we describe the isolation of the  opgE  gene encoding the phosphoethanolamine transferase by a screen previously used for the isolation of the  opgB  gene encoding the phosphoglycerol transferase. Both genes show structural and functional similarities without sequence similarity.

Biosynthesis of Osmoregulated Periplasmic Glucans inEscherichia coli: The Phosphoethanolamine Transferase Is Encoded byopgE