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In our early experiments, the chloroform extract of  D. crassirhizoma  was demonstrated to contain the highest concentrations of total phloroglucinols among several extract fractions and possessed the most effective nematicidal activity. This study aimed to ascertain the ultrastructural changes in  M. incognita  after treatment with a  D. crassirhizoma  chloroform extract at 1 mg · mL −1  for 24 h. It was found that the extract exhibited significant destructive effects on the worm's ultrastructure and caused distinctive damage to body surfaces and internal structures. These results will contribute to a deeper understanding of the nematicidal mechanism of  D. crassirhizoma , as well as in the design of efficient bionematicides.

Effects of Chloroform Extract ofDryopteris crassirhizomaon the Ultramicroscopic Structures ofMeloidogyne incognita