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Cistus heterophyllus  subsp.  carthaginensis  is an endemic and endangered species from the SE Mediterranean coastal region of Spain. Within the framework of the efforts aiming to species conservation,  in vitro  culture techniques could be of interest. The aim of this study was to evaluate the antioxidant capacity of  C. heterophyllus  shoot cultures as a possible marker of  in vitro  performance. The effects of five different basal salt formulations and cytokinin levels on  in vitro  performance and antioxidant capacity were examined. K + /Na +  and Ca 2+ /Na +  ratios initially present in culture media greatly affected the antioxidant capacity (the lower the ratios the higher the antioxidant capacity). Increasing concentrations of BA resulted in higher antioxidant capacity. The results obtained point to antioxidant capacity as being a marker of incidence of stress conditions in  in vitro  cultured  C. heterophyllus . A good correlation was found between antioxidant capacity and total soluble phenolics present in  Cistus  extracts. Catechin was identified in all the extracts and its levels were found to change parallel to the antioxidant capacity, pointing to a prominent role played by this flavonoid in  C. heterophyllus  defence against oxidative stress, which in turn affects the  in vitro  performance of this species.

Antioxidant Capacity as a Marker for Assessing theIn VitroPerformance of the EndangeredCistus heterophyllus