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MicroRNAs (miRNAs) are small noncoding RNAs which repress gene expression at the posttranscriptional level. In this study, an expressed sequence tag (EST)-based combined method was applied for the detection of miRNAs in  Macaca fascicularis  which is used as a model animal extensively in medical experiments, particularly those involved with neuroscience and disease. Initially, previously known miRNA sequences from metazoans were used to blast with the EST databases of  Macaca fascicularis , and then a range of filtering criteria was conducted to remove some pseudo ones. At last a total of 8 novel conserved miRNAs were identified; their functions were further predicted and analyzed. Together, our study provides insight into miRNAs and their functions in  Macaca fascicularis , indicating that the EST analysis is an efficient and affordable alternative approach for identifying novel miRNA candidates.

Identification and Characterization of MicroRNAs inMacaca fascicularisby EST Analysis