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To identify effective herb to treat obesity, we screened 115 herbal extracts for inhibition of porcine pancreatic lipase (triacylg-ycerol acylhydrolase, EC 3.1.1.3) activity  in vitro . Of the extracts tested,  Cudrania tricuspidata  leaves exhibited the most pronounced inhibitory effect on lipase activity with an IC 50  value of 9.91   μ  g/mL. Antilipid absorption effects of  C. tricuspidata  leaves were examined in rats after oral administration of lipid emulsions containing 50 or 250 mg   C. tricuspidata /kg body weight. Plasma triacylglycerol levels 2 h after the oral administration of emulsions containing  C. tricuspidata  were significantly reduced compared to the untreated group ( P  < 0.05). These results suggest that  C. tricuspidata  leaves may be useful for the treatment of obesity.

Inhibitory Activities ofCudrania tricuspidataLeaves on Pancreatic LipaseIn Vitroand LipolysisIn Vivo